We are able to use different expression systems like E. coli, yeasts (e.g. Pichia), or even mammalian cell lines for the expression of the proteins.
We are optimizing the expression conditions and therefore allowing the synthesis of proteins different scales – from reaction tubes and culture flasks to bioreactor. All Standard Cell Culture Equipment is available.
Isolation of cells
- Centrifugation, batch and flow-through
- High pressure homogenizer
- Bead mills
- French press
Clarifications and removal of cell debris, conditioning
- Precipitation of proteins
- Aquous two phase systems
- Chromatographic systems for the purification of proteins (ÄKTApurifier, ÄKTAexplorer, Smart-system)
- SDS-PAGE, IEF, 2-D-electrophoresis, western blotting
- Electrospray with linear ion trap)